FIG. 5.

HPLC chromatograms of radiolabeled ¹²⁵I-Ang-(1–12) substrate and its hydrolytic products generation by ARPE-19 cells. HPLC analysis of the hydrolytic products generated from ¹²⁵I-Ang-(1–12) substrate by solubilized plasma membranes in the absence of both ACE and chymase inhibitors. The HPLC chromatograms, represent the hydrolytic products are generated in presence of ACE and chymase inhibitors (panel A) and in the absence of both inhibitors (panel B). Details are in Methods section. ACE, angiotensin-converting enzyme.