Skip to main content
. 2023 May 3;24(9):8208. doi: 10.3390/ijms24098208

Figure 1.

Figure 1

Transcription of PeGLABRA3 and PePLDδ in salt-stressed Populus euphratica. (A–D) PePLDδ and PeGLABRA3 transcription in NaCl-stressed P. euphratica. Populus euphratica seedlings were exposed to 0 or 200 mM of NaCl for 48 h. Leaves and roots were sampled at 0, 1, 3, 6, 12, 24, and 48 h for RT-qPCR analysis. (E,F) PeGLABRA3 and PePLDδ transcription in PeGLABRA3-silenced P. euphratica. Agrobacterium carrying the TRV1 empty vector and TRV2-PeGLABRA3 vector were injected into P. euphratica leaves. After 45 days of virus-induced gene silencing (VIGS), the P. euphratica seedlings were subjected to 200 mM of NaCl for 24 h. Leaves were sampled from TRV-infected P. euphratica for RT-qPCR analysis. Populus euphratica PeACT7 was used as an internal reference. Primers for PeGLABRA3, PePLDδ, and PeACT7 are shown in Supplementary Table S1. Each point (A–D) or column (E,F) represents the average of three independent replicates, and the error bar represents the standard error of the mean. * p < 0.05, ** p < 0.01.