Multiple sequence alignment and phylogenetic analysis of Populus euphratica PeGLABRA3. (A) Multiple sequence alignment of PeGLABRA3 with GLABRA3 from different species. Black shading indicates identical amino acid residues and blue and pink shadings indicate conserved amino acids, respectively. (B) A phylogenetic relationship between PeGLABRA3 and GLABRA3 proteins from other different species. The phylogenetic tree was constructed using the nearest neighbor joining method with MEGA 7.0 software. At, Arabidopsis thaliana; Nt, Nicotiana tabacum; Pe, Populus euphratica; Pt, Populus trichocarpa; Os, Oryza sativa; Gm, Glycine max. GLABRA3 accession numbers for different species are shown in Supplementary Table S2.