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The luciferase reporter assay (LRA) validated the PeGLABRA3 interaction with the PePLDδ promoter. Nicotiana tabacum leaves were co-transformed with Agrobacterium strains containing (1) PeGLABRA3-62-SK + pGreenII 0800-LUC, (2) PeGLABRA3-62-SK + PePLDδ-pro-LUC, (3) PePLDδ-pro-LUC + pGreenII 62-SK, or (4) pGreenII 0800-LUC + pGreenII 62-SK. The LRA was repeated three times and representative luciferin luminescence images are shown.
