Skip to main content
. 2023 Apr 30;24(9):8094. doi: 10.3390/ijms24098094

Figure 3.

Figure 3

p90RSK regulates PDGF-BB-induced cell migration of VSMCs. (A) Serum starved VSMCs grown near confluent were wounded and then treated for 1 h with either 2 or 5 μM of FMK or BI-D1870, followed by treatment with 2 ng/mL of PDGF-BB for 24 h. The wound area was observed at different time points as indicated and recorded under a phase-contrast microscope. ** p < 0.01 vs. cells treated with PDGF-BB. (B) The Transwell system was used for the selective migration assay. VSMCs were pretreated for 1 h with various concentrations of either FMK or BI-D1870, followed by treatment with 2 ng/mL of PDGF-BB for 24 h. The number of migrated cells to the lower chamber was enumerated under the light microscope. ** p < 0.01 vs. vehicle control (lane 1), ## p < 0.01 vs. cells treated with PDGF-BB (lane 2). (C) VSMCs grown near confluent were transduced either with Ad-WT-p90RSK or Ad-LacZ as a control for 24 h, and then serum-starved cells were wounded. The wound area was observed at different time points as indicated and recorded under a phase-contrast microscope. The data are representative of the three independent experiments. Scale bar, 100 μm. * p < 0.05, ** p < 0.01 vs. vehicle control (Ad-LacZ).