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. 2023 Apr 29;24(9):8060. doi: 10.3390/ijms24098060

Figure 7.

Figure 7

Induction in HPV16 and HIV1-specific antibodies and T-cell responses after L1:P18I10 VLP immunization in BALB/c mice. (A) Immunization schedule. Eight mice (male n = 4 and female n = 4 per group) in each group were immunized intramuscularly (i.m.) twice with either 10 μg of L1:P18I10 VLPs or 10 μg of Gardasil-9 (HPV16 L1 VLPs) vaccines. The homologous prime-boost interval was 2 weeks. The end point of this trial was on day 28. Sera and spleens were collected for ELISA and ELISpot assays, respectively. (B,C) L1 and P18I10-specific antibodies induced by L1:P18I10 VLPs. ELISA assay was performed to analyze anti-HPV16 L1 and anti-HIV1 P18I10 IgG induced by L1:P18I10 VLPs or Gardasil-9 in BALB/c mice. One group of mouse was immunized with only PBS buffer as a naïve group (negative control, without VLP) to set up the cutpoint. Simple linear regression test was carried out to compare the line difference between two groups. ns not significant; ** p < 0.01. (D,E) L1 and P18I10-specific T-cell responses induced by L1:P18I10 VLPs. IFN-γ ELISpot was performed to measure the frequency of IFN-γ secreting splenocytes after stimulation with HPV16 L1 VLP and P18I10 peptide induced by L1:P18I10 VLPs or Gardasil-9 in BALB/c mice. Data are shown as median ± S.D. Unpaired T test was performed to compare differences between groups. ns not significant; * p < 0.05; *** p < 0.001.