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. 2023 Apr 29;24(9):8060. doi: 10.3390/ijms24098060

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Figure A2 — Detection of BEVS/IC-derived L1:P18I10 VLPs in CsCl density gradient. BEVS/IC-derived L1:P18I10 VLPs were partially purified by a two-step sucrose cushion (SC) and subsequently fractionated by CsCl gradient. The CsCl gradient was fractionated from the top of the tube (F1-F15, 400 μL per fraction). Fraction 1 corresponds to the top of the tube. The signal of L1:P18I10 VLPs in each fraction was detected by dot blot, using anti-HPV16 L1 mAb. The HPV16 L1 VLPs were used as a positive control. CsCl-purified VLPs could be heterogeneous in size because of the broken particles and presented as multiple bands.