Table 1.
Purification profiles of L1:P18I10 VLPs.
| Method | Purification Stages | Total HCP (μg) a | HCP Removal (%) | Total L1 (μg) b | Recovery (%) | Purity (%) d |
|---|---|---|---|---|---|---|
| 1 Traditional |
BEVS/IC CCL |
2637.4 | - | 109.4 | 100 | 4 |
| Ultracentrifugation | ||||||
| Step-1: SC | 264.5 | 90 | 16.6 | 15 | 6 | |
| Step-2: CsCl | 12.0 | 99 | 11.9 | 11 | 99 | |
| 2 New |
293F cell expression CCL |
1848.5 | - | 33.3 | 100 | 2 |
| Chromatography | ||||||
| Step-1: CEC | 649.2 | 65 | 21.5 | 65 | 3 | |
| Step-2: SEC | 193.9 | 90 | 19.4 c | 58 | 10 | |
| Step-3: H-AC | 24.7 | 98 | 18.8 c | 56 | 76 |
a: Determined by BCA assay; b: Determined by densitometry of Western blot; c: Determined by ELISA. d: Determined by a ratio of total L1 to total HCP. Abbreviations: CCL: clarified cell lysate; SC: sucrose cushion; CsCl: CsCl density gradient; CEC: cation exchange chromatography; SEC: size exclusion chromatography; H-AC: heparin-affinity chromatography.