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Graphical scheme depicting the underlying mechanisms in different LPS detection methods. From left to right: rabbit pyrogen test (RPT), monocyte activation test (MAT), gel clot limulus amebocyte lysate (LAL) assay and its variants (e.g., chromogenic LAL), EndoLISA, and, ultimately, the TLR4-NF-κB-luciferase (TLR4-NF-κB-luc) reporter gene assay.
