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. 2023 May 7;24(9):8411. doi: 10.3390/ijms24098411

Figure 2.

Figure 2

Determination of C44Mab-6 epitope by ELISA. The synthesized peptides, which cover the CD44v3–10 extracellular domain, were immobilized on immunoplates. The plates were incubated with C44Mab-6, followed by incubation with peroxidase-conjugated anti-mouse immunoglobulins. Optical density was measured at 655 nm. The CD44 p231–250 sequence (AGWEPNEENEDERDRHLSFS) corresponds to the variant 3-encoded sequence. ELISA: enzyme-linked immunosorbent assay. NC: negative control (solvent; DMSO in PBS).