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The binding affinity of C44Mab-6 to CD44-expressing cells. CHO/CD44v3–10 (A), COLO205 (B), and HSC-3 (C) cells were suspended in C44Mab-6 at the indicated concentrations. Cells were incubated with Alexa Fluor 488-conjugated secondary antibody. Fluorescence data were collected, and the apparent dissociation constant (KD) was calculated by using GraphPad Prism 8.
