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. 2023 May 5;24(9):8322. doi: 10.3390/ijms24098322

Figure 1.

Figure 1

The timeline for induction of MetS-associated NAFLD and periodontitis and treatment with imipramine. Six weeks old male C57BL/6 mice were fed high-fat diet (HFD) for 20 weeks to induce metabolic syndrome (MetS) and MetS-associated nonalcoholic steatohepatitis (NASH). Starting from Week 13, part of the mice were treated with imipramine for eight weeks to inhibit acid sphingomyelinase (ASMase). Starting from Week 13, part of the mice were treated with antibiotics for seven days for the preparation of oral P. gingivalis inoculation. In Week 15, part of the mice received P. gingivalis inoculation to induce periodontitis. All mice were sacrificed at the end of 20 weeks.