Figure 3.

The effect of HFD feeding, oral P. gingivalis (Pg) inoculation, and imipramine treatment on alveolar bone loss. C57BL/6 mice were fed HFD for 20 weeks to induce MetS and NAFLD and inoculated orally with Pg in the 15th week to induce periodontitis. Part of the mice were also treated with imipramine in the last eight weeks. After the feeding and treatments, the maxillae were scanned with micro-computed tomography (mCT), and the bone volume fraction was determined (A). In addition, the liner distances from CEJ to ABC for the first and second molars were also measured. Representative mCT images for eight groups were shown (B), and the CEJ-ABC distances were presented as folds of the LFD-fed control mice (C). The locations of the CEJ and ABC and the distance between CEJ and ABC were shown (D). The data are mean ± SD (n = 12).