Figure 6.

The effect of HFD feeding, oral P. gingivalis (Pg) inoculation, and imipramine treatment on hepatic steatosis. C57BL/6 mice were fed HFD to induce MetS/NAFLD and orally inoculated with Pg to induce periodontitis. Part of the mice were also treated with imipramine. At the end of the study, the livers of the mice were dissected, sectioned, and stained with Oil Red O. (A) Representative images of liver sections with Oil Red O staining. (B) The positively stained areas of Oil Red O staining in tissue sections were quantified and compared among all groups. Scale bar = 100 μm. The data are presented as means ± SD (n = 12).