Figure 7.

The effect of HFD feeding, oral P. gingivalis (Pg) inoculation, and imipramine treatment on hepatic inflammation. C57BL/6 mice were fed HFD to induce MetS/NAFLD and orally inoculated with Pg to induce periodontitis. Part of the mice were also treated with imipramine. At the end of the study, the livers of the mice were dissected, sectioned, and subjected to F4/80 immunostaining. (A) Representative images of liver sections with F4/80 immunostaining. (B) The positively stained areas of F4/80 immunostaining were quantified and compared among all groups. Scale bar = 100 μm. The data are presented as means ± SD (n = 12).