Figure 8.

The effect of HFD feeding, oral P. gingivalis (Pg) inoculation, and imipramine treatment on hepatic fibrosis. C57BL/6 mice were fed HFD to induce MetS/NAFLD and orally inoculated with Pg to induce periodontitis. Part of the mice were also treated with imipramine. At the end of the study, the livers of the mice were dissected, sectioned, and stained with Sirius Red. (A) Representative images of liver sections with Sirius Red staining. (B) The positively stained areas of Sirius Red staining were quantified and compared among all groups. Scale bar = 100 μm. The data are presented as means ± SD (n = 12).