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. 2023 May 2;24(9):8141. doi: 10.3390/ijms24098141

Figure 5.

Figure 5

MYCN depletion induces expression of NOTCH target genes. (A) In silico expression analysis of HEY1 and HEYL NOTCH target genes in 39 human NB cell lines. (B) In silico analysis of mRNA levels of NOTCH target genes HES1, HES4, HEY1, and HEYL in primary NB tumors. (C) Kaplan–Meier-analysis overall survival curve from the Tumor Neuroblastoma-SEQC-498 study based on mRNA expression of NOTCH target genes HES1, HES4, HEY1, and HEYL. (D,E) Expression of HES1, HES4, HEY1, and HEYL in response to 1 µg/mL doxycycline in (D) TET21N cell line or (E) KELLY cell line following 0.4 µM MYCMI-7 treatment. Cell lines were treated with doxycycline or MYCMI-7 for 72 h. DMSO was used as control treatment. Statistical significance was calculated using (A) unpaired Student t-test in panel, and (B) one-way ANOVA in panel. (C) Statistical analysis was performed using Bonferroni correction of raw p-values available in panel. For qPCR, p-value was calculated using two-tailed, unpaired Student t-test. * p-value ≤ 0.05; *** p-value ≤ 0.001.