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. 2023 May 2;24(9):8160. doi: 10.3390/ijms24098160

Figure 3.

Figure 3

Increasing EAE-induced inflammatory cells at day 10 (peak disease). Frozen cross sections of spinal cords from CFA-injected control mice at day 10 post-immunization (1st episode, (A–C)) and PLP-induced EAE mice at day 10 (D–F,J) and day 20 after PLP-immunization (2nd episode, (G–I,K)) were stained with anti-GFAP (a marker for astrocytes, (A,D,G,J,K), green), anti-Iba1 (a marker for microglia/macrophage, (B,E,H,J,K), red) antibodies with DAPI ((C,F,I–K), blue). Merged images are shown (C,F,I–K). Images with a high-power magnification of panels (F,I) are shown (J,K). Scar bars: 500 μm.