Figure 5.

EAE-induced angiogenesis in the RR-EAE and the resistant mice. Frozen cross sections of spinal cords from the RR-EAE mice were stained with anti-CD31 antibody (a marker for angiogenesis, green). These sections were also stained with DAPI (blue). Each time course (control (A), day 10 (d10, (B)), day 20 (d20, (C)) are shown. Representative results from RR-EAE ((D), relapse (+)) and the resistant ((E), relapse (−)) are also shown. White arrowheads show a representative angiogenesis stained with anti-CD31 antibody. Panel (F) revealed that the number of CD31+ve cells in the RR-EAE, but not in the resistant mice, were significantly increased from remitting to relapse phase. ** p < 0.01 (vs. control), ## p < 0.01 (vs. relapse (+)) are shown. Scar bar: 50 μm.