Figure 8.

Higher expression of EAAT2 was observed in the RR-EAE mice rather than the resistant mice. Frozen cross sections of spinal cords from the RR-EAE (relapse (+)) (D–I) and the resistant (relapse (−)) mice (A–C) were stained with anti-EAAT2 (a glutamate transporter on astrocytes; (A,D,G), green) and anti-GFAP ((B,E,H), red) antibodies. These sections were also stained with DAPI (blue). Merged images are shown (C,F,I). Images with a high-power magnification of panels (D–F) are shown (G–I). Scar bars: 50 μm.