Figure 11.

21.5 kDa isoform of MBP during relapsing phase was degenerated in RR-EAE. RR-EAE and resistant mice were perfused and immunostain was performed with Frozen cross sections of spinal cords from the RR-EAE (relapse (+)) (A–C) and the resistant (relapse (−)) mice (D–F) were stained with Fluoromyelin ((A,D), red) and anti-7D2 (a marker for 21.5 kDa isoform of MBP; (B,E), green) antibody. These sections were also stained with DAPI (blue). Merged images are shown (C,F). Scar bar: 50 μm. (G) Immunoblot analysis stained with anti-SMI-94R (a marker for MBP), anti-7D2 antibody, anti-QD9 antibody (a marker for degenerating MBP), and anti-GAPDH antibody (as an internal control). Note that anti-7D2 antibody specifically recognizes only 21.5 kDa isoform of MBP.