Skip to main content
. 2023 May 6;24(9):8379. doi: 10.3390/ijms24098379

Figure 3.

Figure 3

Profibrotic conditions potentiate the radical scavenging functionality of vitamin C. iROS formation is upregulated in a timely manner (A). Vitamin C downregulates iROS formation at the late incubation time (B). Similarly, mROS follows a decay trend in time (C), and it vanishes even more in the presence of vitamin C (D). Likewise, iROS and mROS were detected via DHE–mitoSOX–DAPI co-staining of primary cardiac fibroblasts (10,000 cells/well). Error bars indicate standard error of the mean; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. n = 3 experiments per condition. Fbn (Fibronectin); Coll (Collagen); mROS (mitochondrial reactive oxygen species); iROS (intracellular reactive oxygen species).