Table 1.
List of primer sequences.
| Genes | Primer Sequences | Product Length | Annealing Temp. (°C) | GenBank Numbers |
|---|---|---|---|---|
| Col1a1 | 5′-GCTCCTCTTAGGGGCCACT (Forward) | 103 bp | 60 °C | NM_ 007742.4 |
| 5′-CCACGTCTCACCATTGGGG (Reverse) | ||||
| Col3a1 | 5′-TCTGAGCTGCTTCTTCCTCTCT (Forward) | 98 bp | 60 °C | NM_ 009930.2 |
| 5′-GAAGAAACCAGGTTCCACTTTG(Reverse) | ||||
| Col5a1 | 5′-GCTACTCCTGTTCCTGCTGC (Forward) | 100 bp | 60 °C | NM_ 006497644.5 |
| 5′-TGAGGGCAAATTGTGAAAATC (Reverse) | ||||
| β-actin | 5′-AGCCATGTACGTAGCCATCC (Forward) | 228 bp | 60 °C | NM_ 007393.5 |
| 5′-CTCTCAGCTGTGGTGGTGAA (Reverse) |