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Route of transepithelial permeation of fluorescein-labeled 40-kDa dextran (fixable; green fluorescence) and rhodamine-labeled 45-kDa ovalbumin (red fluorescence) across MDCK-1 cell monolayers infected with yopE mutant (a, merged dextran and ovalbumin images) or wild-type (b, merged dextran and ovalbumin images; c, dextran image; d, ovalbumin image) Y. pseudotuberculosis, as assessed by horizontal confocal sectioning. Bars, 5 μm.
