FIG. 1.

LSCM demonstrates that the characteristic cytokeratin-staining pattern of the tissue biopsies has been retained in the respective primary cervical epithelial cell cultures. Sectioned tissue biopsies (left column, ×20 magnification) and primary cervical epithelial cell monolayers (right column, oil immersion, ×60 magnification) were incubated with an FITC-conjugated antibody to the noted specific cytokeratin. Ethidium bromide was used to counterstain the tissue sections. (A) As can be seen, endocervical cells were labeled intensely with antibody 8.12, which is specific for type I cytokeratins 13, 15, and 16. (C) The labeling of endocervical cells with an antibody specific for type II cytokeratin 4 was considerably less intense, and it was not uniformly distributed. Ectocervical cells were labeled positive with an antibody specific for cytokeratins 13, 15, and 16 (B) and cytokeratin 4 (D).