FIG. 2.

Constitutive and regulated expression of human β-defensin mRNA in AGS cells in response to proinflammatory agonists. AGS cells were stimulated with IL-1α, TNF-α, IFN-γ, or LPS (2 or 10 μg/ml); infected with enteroinvasive E. coli O29:NM, E. coli DH5α, S. enterica serovar Dublin or S. bovis; or left untreated (control) as indicated. Total RNA was isolated, and hBD-1, hBD-2, and β-actin were amplified by RT-PCR.