Supplementation of C17:0 leads to the highest induction of OCFA in liver, plasma and white adipose tissue. C57BL/6JRj mice were fed semi-synthetic low-fat diet (LFD), high-fat diet (HFD, depicted in grey) or HFD supplemented with either 14% milk fat (HFMF; depicted in light yellow) or 5% heptadecanoic acid (HFC17, depicted in dark yellow) for 20 weeks. Composition of long-chain fatty acid (LCFA) phospholipids in (A) liver, (B) plasma and (C) epididymal white adipose tissue (eWAT) after 20 weeks of intervention, n = 7−8. Results are expressed as area percentage of each fatty acid relative to the total area of all detected fatty acids. (D) Principal component (PC) analysis showing the score plot of data including hepatic OCFA formation. Data are mean + SEM, * p < 0.05; ** p < 0.01, *** p < 0.001. OCFA, odd-chain fatty acids; ω3FA, omega-3 fatty acids; ω6FA, omega-6 fatty acids.