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. 2023 Apr 24;15(9):2052. doi: 10.3390/nu15092052

Figure 3.

Figure 3

Long-term supplementation of C17:0 elicits negligible effects on insulin sensitivity. C57BL/6JRj mice were fed semi-synthetic low-fat diet (LFD, dotted line), high-fat diet (HFD, depicted in grey) or HFD supplemented with either 14% milk fat (HFMF; depicted in light yellow) or 5% heptadecanoic acid (HFC17, depicted in dark yellow) for 20 weeks. (A) Glucose (Glc) and insulin (Ins) concentrations after 16 h of fasting, n = 6. (B) Time-dependent blood glucose (BG) levels and (C) concentrations of insulin after oral glucose gavage (2 g/kg body weight) in week 16 and corresponding incremental area under the curves (iAUC), n = 6. (D) Time-dependent BG levels after i.p. insulin (0.75 U/kg body weight) in week 16 and corresponding iAUC, n = 6. (E) Relative analysis of immunohistochemical (IHC)-stained insulin in whole pancreatic sections and representative images, n = 3. Data are mean +/− SEM, * p < 0.05. OGTT, oral glucose tolerance test; ITT, insulin tolerance test.