Figure 6.

The antioxidant effect of the CS and CU extracts was evaluated using DCFH-DA assay on normal fibroblast cells (BJ). Cells pre-exposed for 24 h to the extracts (100, 200, and 300 µg/mL) or NAC (20 mM), were further incubated with 50 µM DCFH-DA. The antioxidant properties of the CA extracts were measured after 2 h in non-stimulated (HBSS) and stimulated conditions (250 µM H₂O₂). The data are presented as relative means ± standard deviations of three biological replicates (six technical replicates/biological replicate) in comparison with the negative control (100%). Different letters (non-stimulated conditions (a–d) and stimulated conditions (A–D)) mark significant differences (ANOVA + Holm-Sidak post hoc, p < 0.05).