TABLE 2.
Internalization of A. actinomycetemcomitans by HUVEC
| A. actinomycetemcomitans strain | CV3988 concn (μM) | No. of CFU/ml (mean ± SD)
|
% of CFU
|
||
|---|---|---|---|---|---|
| Bound + internalized | Internalizeda | Compared to controlb | Internalizedc | ||
| D045D-40 | 0 | 3,250,000 ± 926,427 | 36,650 ± 5,400 | 1.13 | |
| 1 | 2,580,000 ± 473,568 | 28,400 ± 2,861 | 77 | 1.1 | |
| 25 | 1,870,000 ± 441,362 | 6,350 ± 1,204 | 17 | 0.34 | |
| 50 | 1,745,000 ± 634,849 | 1,655 ± 375 | 5 | 0.09 | |
| DB03A-42 | 0 | 33,500 ± 15,264 | 0 | 0 | 0 |
a
Cultures of HUVEC containing A. actinomycetemcomitans were washed and treated with gentamicin. Subsequently, HUVEC were lysed and bacteria were plated to determine the number of CFU remaining.
b
Control cultures contained no CV3988.
c
CFU remaining following gentamicin treatment/CFU without gentamicin treatment × 100.