TABLE 2.

Fluorescence-activated cell sorter analysis of CD4⁺ Vβ T-cell subsets induced by P. intermedia^a

Culture	% of cells expressing:
	Vβ1	Vβ8	Vβ12	Vβ17	Vβ22
PBMC alone	3.1 ± 0.1	2.4 ± 0.1	2.8 ± 0.2	3.5 ± 0.1	4.0 ± 0.2
PBMC + strain 17	2.7 ± 0.1	4.3 ± 0.0	6.2 ± 0.1	7.4 ± 0.1	4.2 ± 0.2
PBMC + strain 25611	2.8 ± 0.0	2.5 ± 0.1	5.4 ± 0.2	3.2 ± 0.2	3.0 ± 0.1
PBMC + E. coli	3.0 ± 0.1	2.2 ± 0.1	2.4 ± 0.1	3.1 ± 0.2	4.6 ± 0.3

^aPBMC were cultured with medium or in the presence of the bacteria indicated for 72 h as described in the legend for Fig. 3. Cells were washed, resuspended in FACS buffer (PBS containing 5% fetal bovine serum and 0.1% sodium azide), and adjusted to 10⁷ cells/ml. Cells were then stained with fluorescein-labeled anti-CD4 for 1 h. After being washed in FACS buffer, cells were stained with rhodamine-labeled anti-Vβ antibodies. Cells were washed in FACS buffer, and Vβ subsets of CD4⁺ T cells were quantified using a Becton Dickinson FACSort. Values shown are the percentages of CD4⁺ T cells that double-stained for the indicated Vβ. The data are representative of three experiments, each with a different donor.