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. 2023 Apr 24;15(9):2044. doi: 10.3390/nu15092044

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The expressions of cytokines and apoptosis-related proteins are enhanced by λ-COS. (a) The effect of λ-COS on the concentrations of TNF-α and IFN-γ in mouse serum. The blood samples were collected from the eyeballs of mice. The levels of cytokines in the serum samples were detected by ELISA. (b) The expressions of cleaved-caspase-8 and Bcl-2 in tumor tissues of all groups. The expressions of the apoptosis-related proteins were evaluated via immunohistochemical detection. * p < 0.05 and ** p < 0.01 versus the vehicle group. ^# p < 0.05 and ^## p < 0.01 versus the 5-FU group. (c) The mean densities of cleaved-caspase-8 and Bcl-2 in tumor tissues of all groups were evaluated by using ImageJ software. Bar = 20 μm.