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. 2023 Apr 20;12:e80900. doi: 10.7554/eLife.80900

Figure 2. Diphtheria toxin (DT) administration results in an acute lung injury phenotype.

Figure 2.

(A) Representative examples of Evans blue staining of lungs from binary transgenic mice. (B) Summary data showing a marked increase in Evans Blue lung content in binary transgenic mice treated with DT (10 ng) or saline at 3 and 7 days post-treatment (dpt). (C) Representative histological lung sections (H&E staining) from binary transgenic mice at 3 and 7 days post intra-tracheal (IT) administration of DT or saline with the corresponding summary data (D) for a validated lung injury score. (E) Representative examples of flow cytometry plots showing gating strategy for assessing CD11b/Ly6G positive leukocytes in lungs from binary transgenic mice 3 days after IT delivery of DT or saline with summary data (F) at 3 and 7 days post-treatment (dpt). Data represented as mean ± SEM. An unpaired multiple t-tests with Holm-Sidak multiple comparisons method with alpha (0.05) was used for the analysis of data presented in panels B and F, whereas one-way ANOVA corrected for multiple comparisons with Dunnett was conducted for panels D. n=3-4 biological replicates per group, *=p<0.05; **=p<0.01; ***=p<0.005. Scale bar is 50μm in panel C.