Table 4.
Predicted functional genes from the cecal microbiome of PbOAc and SRM 2710a treated mice compared to the cecal microbiome from animals that received no Pb or other metals. The 16 S sequences from the top 20 OTUs contributing most to dissimilarity at the highest dose of PbOAc (25 mg/kg) and SRM 2710a (30 mg/kg) were analyzed using the CLC Genomics Workbench (22.0.2; Microbial Genomics Module) as described. MetaCyc (metacyc.org) (Caspi et al., 2020) and Transform Column-All Ancestors of Entity were used to identify super pathways.
| Predicted Function | PbOAc | SRM 2710a | Control |
|---|---|---|---|
|
|
|
|
|
| Average Gene Abundance | Average Gene Abundance | Average Gene Abundance | |
|
| |||
| Metal Resistance | |||
| Siderophore | 1082 | 144 | 7 |
| Arsenic | 52 | 217 | 0 |
| Mercury | 1 | 431 | 14 |
| Antibiotic Resistance | |||
| vancomycin | 19,984 | 13,963 | 22,588 |
| vancomycin | 11,792 | 7333 | 11,545 |
| polymyxin | 310 | 927 | 38 |
| jadomycin | 58,181 | 89,271 | 31,767 |
| phenazine compounds | 1649 | 2931 | 578 |
| tetracenomycin C | 176 | 465 | 756 |
| Biosynthesis | |||
| Carbohydrates | 219,846 | 242,655 | 208,515 |
| Lipid | 151,886 | 176,104 | 106,770 |
| Degradation | |||
| Carbohydrates | 935,609 | 1668,098 | 895,936 |
| Fatty Acid & Lipid | 84,878 | 113,694 | 50,863 |