Extended Data Fig. 3. Fitted ED₅₀ values are well-correlated with orthogonally calculated measures of sensitivity.

For all SOX9-dependent REs with good Hill equation fits (p < 0.05 for both ED₅₀ and Hill exponent), correlation between either ED₅₀ (left, Spearman ρ −0.961) or Hill exponent (right, Spearman ρ −0.457) and buffering index calculated at 50% SOX9 dosage. See Methods for details on calculation of buffering index – 0 means no buffering (effect of 100 to 50% SOX9 dosage on RE accessibility is 50% of effect of 100 to 0% SOX9 dosage), 100 means full buffering (no effect of 100 to 50% SOX9 dosage on RE accessibility, but substantial effect of 100 to 0% SOX9 dosage).