Extended Data Fig. 1. Endogenous C-terminal tagging of SOX9.
(a) Genome editing of WT hESCs to derive SOX9-tagged hESCs. Top, schematic depicting primer (arrow) locations for clonal genotyping of the SOX9 locus. SOX9LHA-FKBP12FV36-mNG-V5-SOX9RHA is the full homology-directed repair template provided by AAV6, so the right primer is located outside the homology arm. Bottom, agarose gel images of PCR using depicted primers on 48 analyzed hESC clones nucleofected with SOX9 sgRNA-Cas9 RNP and transduced with tag-containing AAV6. * clones with bi-allelic knock-in. (b) Single-cell distributions of mNeonGreen fluorescence (at least 7,000 cells per histogram) between two SOX9-tagged clones from two CNCC replicates. Representative of two independent experiments.