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. 2023 Apr 5;42(20):1649–1660. doi: 10.1038/s41388-023-02683-w

Fig. 2. Ablation of RAF1 results in cell cycle defects.

Fig. 2

A KI67 staining of spheroids after 5 days in culture. KO pictures are representative of three independent clones/cell line. Scale bars = 200 µm. B Quantification of KI67-positive cells in parental and RAF1 KO spheroids. C 5 days after spheroid formation, spheroids were incubated with 10 µM EdU for 2 h, dissociated, fixed and stained. Cell cycle phases were determined using flow cytometry. RAF1 KO plot represents the three independent RAF1 KO clones for each cell line (n = 3 separate experiments). B, C, *p < 0.05, **p < 0.01, ***p < 0.001, n.s not significant.