Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 5;42(20):1649–1660. doi: 10.1038/s41388-023-02683-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Representative pictures of four patient-derived organoids stably transduced with inducible RAF1 shRNA. Addition of doxycycline (0.5 µg/ml), induces the expression of GFP and of RAF1 shRNA. Scale bars = 250 µm. B Comparison of PDOs volume in Dox-induced/uninduced organoids expressing a control shRNA or two independent RAF1 shRNA. Experiments were performed in triplicate at least three times. C Immunoblot showing RAF, MEK and ERK status in Dox-induced PDOs. Tubulin or GAPDH were used as loading controls. D Patient-derived organoids were treated for 8 days with either DMSO (vehicle) or 1 µM GW5074 prior to end volume determination. The experiment was repeated three times. B, D, *p < 0.05, **p < 0.01, ***p < 0.001, n.s not significant.