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. 2023 May 12;14(5):321. doi: 10.1038/s41419-023-05843-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Representative immunohistochemical images of satellite cells in skeletal muscle showed the expression of Pax-7, and the number of Pax-7⁺ cells in different visual fields in SAMP8 mice was calculated (P_{R1 & PBS} < 0.0001; P_{MSC & PBS} < 0.0001; P_{R1 & MSC} = 0.0006) (scale bar = 25 μm; n = 8 or 10 views per group from 5–6 male mice). C, D The expression of Pax-7 in gastrocnemius muscle of R1, P8-PBS and P8-MSC groups was detected by western blot and statistically analyzed (P_{R1 & PBS} = 0.0691; P_{MSC & PBS} = 0.0001; P_{R1 & MSC} = 0.0008) (n = 3 per group; all data shown as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001).