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. 2023 May 12;14(5):321. doi: 10.1038/s41419-023-05843-8

Fig. 5. hUC-MSCs increased autophagy and delayed myocyte senescence through p16-Rb/p53-p21 axis in SAMP8 mice.

Fig. 5

A, B, C, D The expressions of Lamp2(PR1 & PBS = 0.0151; PMSC & PBS = 0.0243), LC3B II/I (PR1 & PBS = 0.0193; PMSC & PBS = 0.0016), p16(PR1 & PBS = 0.0011; PMSC & PBS = 0.0020) and p53(PR1 & PBS = 0.0001; PMSC & PBS = 0.0001) in muscle cells of R1, P8-PBS and P8-MSC mice were detected by western blot (n = 3 per group; all data shown as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001). E, F Representative immunofluorescence images showed the expression of p16(PR1 & PBS < 0.0001; PMSC & PBS < 0.0001; PR1 & MSC = 0.0221), p53(PR1 & PBS = 0.0005; PMSC & PBS = 0.0003) and p21(PR1 & PBS = 0.0001; PMSC & PBS = 0.0001) in skeletal muscle in R1, P8-PBS and P8-MSC mice (scale bar = 50 μm) and quantified its expression based on the mean fluorescence value in various perspectives of view (n = 8 or 10 views per group from 5–6 male mice; all data shown as mean ± SEM, ***P < 0.001).