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. 2023 May 12;14(5):322. doi: 10.1038/s41419-023-05837-6

Fig. 4. PRMT2 directly activates WNT5A transcription via mediating H3R8me2a enrichment on its promoter.

Fig. 4

A, B Luciferase reporter gene assays detecting WNT5A promoter region activity after knockdown PRMT2 in ACHN and 786-O cells. C, D Luciferase reporter gene assays detecting WNT5A promoter region activity after over-expressing PRMT2 in HEK293T cells. E–H ChIP assays were undertaken in ACHN and 786-O cells after the knockdown of PRMT2 with antibodies against PRMT2 and H3R8me2a. I–L ChIP assays were undertaken in ACHN and 786-O cells after the overexpression of PRMT2 with antibodies against PRMT2 and H3R8me2a. IgG was used for negative control. The enrichment of PRMT2 and H3R8me2a binding to the WNT5A promoter was quantified with qRT-PCR. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.