Fig. 5.

Protein features for prioritizing VUSs. Folding free energy differences (∆∆G_Fold) versus a percent of solvent accessible surface area (SASA) at a variant’s amino acid position, and b model confidence at the variant position for all classified missense variants in the Deafness Variation Database (DVD). Pathogenic and likely pathogenic variants favor buried, high confidence protein regions. c A histogram of the percent SASA for all prioritized VUSs. Similar to known P and LP, the prioritized VUSs are mostly in buried, high confidence protein regions