Table 4.

Average MolProbity refinement statistics for all deafness associated protein models in OtoProtein2 before and after optimization with Force Field X

Optimization	Clash score	Poor rotamers (%)	Favored backbones (%)	Backbone outliers (%)	MolProbity Score
AlphaFold2	20.75	4.32	76.21	15.25	2.86
OtoProtein2	0.11	1.12	93.50	1.05	0.97

A lower clash score, a lower percentage of poor rotamers, a higher percentage of favored backbone phi/psi angles, fewer backbone outliers and lower MolProbity score are each better