Figure 3.

Effect of adhesion between CBCs on blastopore closure

(A) Blastopore and CBC structure.

(B) Coronal section of the blastopore cleared with benzyl alcohol/benzyl benzoate (BABB) solution. Green area, CBC. Scale bar = 100 μm.

(C) Embryos with aluminum foil inserted into the blastopore. Scale bar = 500 μm.

(D) Coronal section image of the blastopore with aluminum foil inserted and cleared with BABB. White dotted line outlines the aluminum foil. Scale bar = 100 μm.

(E-J) Snapshot images from representative time-lapse imaging of fluid excretion in control (E-G; n = 6) and aluminum foil-inserted embryo (H-J; n = 6). Green, cells; magenta, archenteron fluid. Initiation of fluid excretion was set as 0 min. Scale bars = 500 μm.

(K) Representative image of an embryo with glass bead inserted.

(L-M) Magnified view of the dotted area in (K) immediately after removing the bead (L) and 6 s later (M).

(N) Change over time in blastopore opening area (n = 9). Scale bars = 500 μm (K), 100 μm (L, M). n = number of animals used. See also Figure S4 and Videos S5 and S6.