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. 2023 Apr 8;26(5):106585. doi: 10.1016/j.isci.2023.106585

Figure 5.

Figure 5

Decrease in ventral actomyosin contraction during blastopore opening

(A-O) Immunostaining of the blastopore using anti-pMLC and anti-E-cadherin antibodies. Cells were labeled by the injection of 10 kDa Alexa Fluor 647-dextran. Representative transverse section images of the blastopore cleared with BABB at stage 18 (A-C), stage 20-21 (D-F), and excretion stage (G-I). Representative images of the surface (J-L) and sagittal section (M-O) in the ventral end of the blastopore reconstructed from continuous optical sections. Green, pMLC; magenta, E-cadherin; blue, cells; arrowheads, pMLC signals. Scale bars = 10 μm (A-I), 50 μm (M-O).

(P-R) Schematic of transverse section (P), ventral surface (Q), and sagittal section (R) of the blastopore. Green, pMLC signals; Magenta, and blue dotted lines correspond to that of the summary figure S.

(S) Posterior view of the blastopore. (P) and (R) are schematics of the region transected by the magenta and blue dotted lines in (S), respectively. (N) corresponds to the orange square in (S).

(T and U) Ratio of apical width to apical-basal length of epithelial cells at the ventral end of the blastopore. Transverse section; stage 18 (N = 165 cells from 7 embryos), stage 20-21 (N = 137 cells from 5 embryos), excretion stage, (N = 129 cells from 6 embryos). Sagittal section; stage 18 (N = 97 cells from 7 embryos), stage 20-21 (N = 46 cells from 5 embryos), excretion stage (N = 62 cells from 6 embryos). Blue lines, median. ∗∗∗p = 0.0006, ∗∗∗∗p < 0.0001.