Antibodies |
|
Rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) |
Cell Signaling Technology |
Cat # 3671; RRID: AB_330248
|
Mouse anti-E-cadherin |
BD Biosciences |
Cat # 610181; RRID: AB_397580
|
Alexa Fluor 488 goat anti-rabbit IgG (H + L) |
Thermo Fisher Scientific |
Cat # A11029; RRID: AB_2534088
|
Alexa Fluor 546 goat anti-rabbit IgG (H + L) |
Thermo Fisher Scientific |
Cat # A11030; RRID: AB_2534089
|
|
Chemicals, peptides, and recombinant proteins |
|
Dextran, Alexa Fluor™ 488; 10,000 MW |
Thermo Fisher Scientific |
Cat #D22910
|
Dextran, Alexa Fluor™ 568; 10,000 MW |
Thermo Fisher Scientific |
Cat #D22912
|
Dextran, Alexa Fluor™ 647; 10,000 MW |
Thermo Fisher Scientific |
Cat #D22914
|
Alexa Fluor™ 488 Hydrazide |
Thermo Fisher Scientific |
Cat # A10436 |
Phenol red solution |
Sigma-Aldrich |
Cat #P0290 |
Y27632 |
Nacalai Tesque |
Cat # 08945 |
Methylhydrogen silicone fluid (KF-99) |
Shin-Etsu Silicone |
Cat # KF-99-1 |
|
Experimental models: Organisms/strains |
|
Wild type and albino Xenopus laevis frogs |
Hamamatsu Seibutsu Kyozai, Watanabe Zoushoku |
N/A |
|
Software and algorithms |
|
ImageJ/Fiji (ver. 1.53t) |
Schindelin et al. |
RRID: SCR_002285
|
GraphPad Prism 8 (ver. 8.4.3) |
GraphPad Software |
RRID: SCR_002798
|
Leica Application Suite X (ver. 3.5.7) |
Leica Microsystems |
RRID: SCR_013673
|
Labchart 7 |
ADInstruments |
RRID: SCR_001620
|
|
Other |
|
Aluminum foil (d: 5 μm) |
Nilaco |
Cat # AL-013131 |
Tungsten wire (φ: 30 μm) |
Nilaco |
Cat # W-461097 |
950Ag wire (φ: 0.4 mm) |
Comokin |
N/A |
Glass capillary |
Narishige |
Cat # GC-1 |
FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes |
Thermo Fisher Scientific |
Cat #F8888 |