Table 5.

Top 20 proteins ranked in importance measurements in LASSO, Random Forest and XGBoost

Logistic Regression with LASSO penalty			Random Forest			XGBoost
Protein Name	Direction	Percentage	Protein Name	Direction	MDA	Protein Name	Direction	Gain
IL2-ev	Increased	46.4	CRTAC1	Decreased	2.939	CRTAC1	Decreased	0.044
CAMP	Increased	43.2	CCL5-ev	Increased	2.759	CCL5-ev	Increased	0.033
IGHA1	Decreased	40	IGF1	Decreased	2.509	CAMP	Increased	0.033
IGLV1-47	Decreased	36	NGFB-ev	Increased	2.423	IGF1	Decreased	0.022
CRTAC1	Decreased	36	CXCL8-ev	Increased	2.394	IGLV1-47	Decreased	0.021
LRG1	Decreased	30.8	IL2-ev	Increased	2.377	IL2-ev	Increased	0.020
IGF1	Decreased	28.8	CPB2	Increased	2.325	LRG1	Decreased	0.019
PVR	Decreased	24.8	IL12p70-p	Decreased	2.14	CXCL8-ev	Increased	0.018
KNG1	Increased	22.8	IGLV1-47	Decreased	1.897	IL22-p	Decreased	0.015
TUBA1B/A/C	Decreased	7.6	IGLV3-10	Decreased	1.828	TNFα-p	Increased	0.013
IGFALS	Decreased	6	IGFALS	Decreased	1.794	IGFALS	Decreased	0.012
VEGF-ev	Increased	5.2	CAMP	Increased	1.758	IGHA1	Decreased	0.012
IGLV2-11	Decreased	5.2	CCL7-ev	Increased	1.593	GSN	Decreased	0.011
C4BPB	Increased	2.4	LRG1	Decreased	1.486	TUBA1B/A/C	Decreased	0.011
BTD	Decreased	1.2	HBB	Increased	1.366	CXCL10-ev	Decreased	0.011
IL7-p	Decreased	0.4	IL15-ev	Increased	1.337	HBB	Increased	0.010
TNFα-p	Increased	0.4	TUBA1B/A/C	Decreased	1.196	MASP1	Decreased	0.009
IGKV1D-16	Increased	0.4	LCAT	Decreased	1.048	HYI	Decreased	0.009
APOC1	Increased	0.4	IGFBP3	Decreased	1.024	CCL7-ev	Increased	0.009
MBL2	Increased	0.4	PKM	Decreased	1.021	IGLV3-10	Decreased	0.009

Direction is measured relative to controls. Proteins followed by “p” or “ev” come from the plasma and EV cytokines datasets respectively, all other proteins are from the plasma proteomic dataset