Figure 3.

Fucoxanthin treatment inhibited glucose metabolism in ovarian cancer cells. A. The 18F-fludeoxyglucose (F-FDG) uptake was detected in OVACR3 and A2780 cells after fucoxanthin treatment. B. Adenosine triphosphate (ATP) levels were measured using the ATP Assay Kit in OVCAR-3 and A2780 cells. C. Extracellular acidifications rates (ECAR) of OVCAR-3 and A2780 cells was measured with a seahorse Bioanalyzer. D, E. Western blot assay was performed to detect the protein expression levels of glycolysis-related rate limiting enzymes lactate dehydrogenase A (LDHA), hexokinase II (HK2), 3-Phosphoinositide-dependent protein kinase 1 (PDK1). *P<0.05, **P<0.01 and ***P<0.001 vs control.