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. 2023 May 13;23:435. doi: 10.1186/s12885-023-10928-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Cellular Uptake: (a) Confocal microscopy images showing cellular uptake of FITC-SLN by PPCL-192 after incubation for 3 h. The nucleus was stained using the DNA-binding dye Hoechst 33,342 (shown in blue) after cell fixation and the presence of FITC-SLN in cells labeled in green. The merged image exhibits the co-localization of FITC-SLN and Hoechst 33,342 in the nucleus of PPCL-192 cells (Scale bar = 10 µm). b Flow cytometry analysis of PPCL-192 cellular uptake of FITC and FITC-SLN compared to untreated (control) cells after 4 h of incubation. The geometric mean of PPCL-192 cellular uptake of FITC and FITC-SLN. (***p < 0.001 for comparing the geometric mean of untreated, FITC-treated, and FITC-SLN-treated cells) Untreated = negative control; FITC = positive control and FITC-SLN = cells exposed to FITC-SLN, n = 3