Table 1.

Average quality measures of assemblies based on data from Illumina MiSeq, ONT MinION, or both. IL = Illumina only, R9ONLR = R9ONT assemblies polished with ONT reads, R9ONLRSR = R9ONT assemblies additionally polished with Illumina short reads, R9UC = Unicycler assemblies based on R9ONT and Illumina, R10ONLR = R10ONT assemblies polished with ONT reads, R10ONLRSR = R10ONT assemblies polished additionally with Illumina short reads, R10UC = Unicycler assemblies based on R9ONT and Illumina

	Name	#Baseparis	#Contigs	N50 bp	%GC	% Coverage reference genome
Ba. anthracis	IL	5.454.344	85,0	213.938	35,12	99,01
	R9ONLR	5.505.633	3,0	5.229.038	35,25	99,99
	R9ONLRSR	5.506.307	3,7	5.228.860	35,25	99,98
	R9UC	5.504.342	3,3	5.227.886	35,25	99,98
	R10ONLR	5.489.536	2,8	5.228.731	35,26	99,69
	R10ONLRSR	5.489.204	2,8	5.228.416	35,26	99,69
	R10UC	5.504.352	3,5	5.202.007	35,25	99,98
	Ames Ancestor	5.227.419	3,0		35,24
Br. suis	IL	3.306.237	34,0	184.611	57,23	98,82
	R9ONLR	3.328.684	2,0	1.962.140	57,21	99,34
	R9ONLRSR	3.328.513	2,0	1.962.040	57,21	99,34
	R9UC	3.329.933	4,0	1.943.040	57,21	99,29
	R10ONLR	3.328.496	2,0	1.962.244	57,21	99,33
	R10ONLRSR	3.328.496	2,0	1.962.075	57,21	99,33
	R10UC	3.329.930	3,7	1.943.035	57,2	99,29
	1330	3.315.175	2,0		57,25
F. tularensis	IL	1.788.491	103,8	25.987	32,17	94,21
	R9ONLR	1.894.822	1,7	1.892.558	32,17	99,75
	R9ONLRSR	1.894.774	1,7	1.892.510	32,17	99,75
	R9UC	1.876.814	1,3	1.578.270	32,17	98,92
	R10ONLR	1.892.497	1,0	1.892.376	32,16	99,75
	R10ONLRSR	1.892.460	1,0	1.892.460	32,165	99,75
	R10UC	1.871.521	1,5	1.423.518	32,17	98,65
	OSU18	1.895.727	1,0		32,16